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human hcc cell lines hep3b  (ATCC)


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    ATCC human hcc cell lines hep3b
    Human Hcc Cell Lines Hep3b, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hcc+cell+lines+hep3b/Hep+3B2%2E1-7/pm41554703-186-1-16
    Average 96 stars, based on 1140 article reviews
    human hcc cell lines hep3b - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Pan-cancer pervasive upregulation of 3′ UTR splicing drives tumourigenesis
    Article Snippet: .. Human HCC cell lines Hep3B (ATCC: HB-8064) and HepG2 (ATCC: HB-8065) were cultured in DMEM and SNU398 (ATCC: CRL-2233) in RPMI. .. The colon adenocarcinoma (COAD) cell line DLD-1 (Horizon Discovery: HD PAR-086) was cultured in RPMI.

    Article Title: Gut–liver translocation of pathogen Klebsiella pneumoniae promotes hepatocellular carcinoma in mice
    Article Snippet: K. pneumoniae (isolated from tumour tissues of patients with HCC in this study), E. coli (used in Fig. , isolated from tumour tissues of patients with HCC in this study), B. thetaiotaomicron (isolated from tumour tissues of HCC-FMT-treated mice in this study), P. mirabilis (isolated from tumour tissues of HCC-FMT-treated mice in this study), Enterobacter hormaechei (isolated from tumour tissues of HCC-FMT-treated mice in this study), E. coli MG1655 (700926, American Type Culture Collection (ATCC)) and K. oxytoca (DSM5175, DSMZ) were all cultured in brain heart infusion (BHI) broth (024053, Huanan Microbial) at 37 °C under aerobic/anaerobic conditions. .. Both human HCC cell lines Hep3B (HB-8064, ATCC), Huh7 (01042712, Sigma-Aldrich) and normal colonic epithelial cells NCM460 (NCM460D, Incell) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (11965092, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C in a humidified atmosphere of 5% CO 2 . ..

    Article Title: Gut-liver translocation of pathogen Klebsiella pneumoniae promotes hepatocellular carcinoma in mice.
    Article Snippet: K. pneumoniae (isolated from tumour tissues of patients with HCC in this study), E. coli (used in Fig. 4a, isolated from tumour tissues of patients with HCC in this study), B. thetaiotaomicron (isolated from tumour tissues of HCC-FMT-treated mice in this study), P. mirabilis (isolated from tumour tissues of HCC-FMT-treated mice in this study), Enterobacter hormaechei (isolated from tumour tissues of HCC-FMT-treated mice in this study), E. coli MG1655 (700926, American Type Culture Collection (ATCC)) and K. oxytoca (DSM5175, DSMZ) were all cultured in brain heart infusion (BHI) broth (024053, Huanan Microbial) at 37 °C under aerobic/anaerobic conditions. .. Both human HCC cell lines Hep3B (HB-8064, ATCC), Huh7 (01042712, Sigma-Aldrich) and normal colonic epithelial cells NCM460 (NCM460D, Incell) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (11965092, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C in a humidified atmosphere of 5% CO2. ..

    Modification:

    Article Title: Gut–liver translocation of pathogen Klebsiella pneumoniae promotes hepatocellular carcinoma in mice
    Article Snippet: K. pneumoniae (isolated from tumour tissues of patients with HCC in this study), E. coli (used in Fig. , isolated from tumour tissues of patients with HCC in this study), B. thetaiotaomicron (isolated from tumour tissues of HCC-FMT-treated mice in this study), P. mirabilis (isolated from tumour tissues of HCC-FMT-treated mice in this study), Enterobacter hormaechei (isolated from tumour tissues of HCC-FMT-treated mice in this study), E. coli MG1655 (700926, American Type Culture Collection (ATCC)) and K. oxytoca (DSM5175, DSMZ) were all cultured in brain heart infusion (BHI) broth (024053, Huanan Microbial) at 37 °C under aerobic/anaerobic conditions. .. Both human HCC cell lines Hep3B (HB-8064, ATCC), Huh7 (01042712, Sigma-Aldrich) and normal colonic epithelial cells NCM460 (NCM460D, Incell) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (11965092, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C in a humidified atmosphere of 5% CO 2 . ..

    Article Title: Gut-liver translocation of pathogen Klebsiella pneumoniae promotes hepatocellular carcinoma in mice.
    Article Snippet: K. pneumoniae (isolated from tumour tissues of patients with HCC in this study), E. coli (used in Fig. 4a, isolated from tumour tissues of patients with HCC in this study), B. thetaiotaomicron (isolated from tumour tissues of HCC-FMT-treated mice in this study), P. mirabilis (isolated from tumour tissues of HCC-FMT-treated mice in this study), Enterobacter hormaechei (isolated from tumour tissues of HCC-FMT-treated mice in this study), E. coli MG1655 (700926, American Type Culture Collection (ATCC)) and K. oxytoca (DSM5175, DSMZ) were all cultured in brain heart infusion (BHI) broth (024053, Huanan Microbial) at 37 °C under aerobic/anaerobic conditions. .. Both human HCC cell lines Hep3B (HB-8064, ATCC), Huh7 (01042712, Sigma-Aldrich) and normal colonic epithelial cells NCM460 (NCM460D, Incell) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (11965092, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin at 37 °C in a humidified atmosphere of 5% CO2. ..

    Transformation Assay:

    Article Title: XPO1 intensifies sorafenib resistance by stabilizing acetylation of NPM1 and enhancing epithelial-mesenchymal transition in hepatocellular carcinoma.
    Article Snippet: Emerging studies have suggested that exportin-1 (XPO1) plays a pivotal role in hepatocellular carcinoma (HCC).. However, the underlying mechanism of XPO1 in HCC sorafenib resistance remains enigmatic.. The expression of XPO1 in HCC tumor tissues and sorafenib-resistant (SR) cells were analyzed by bioinformatics analysis, immunohistochemistry (IHC) and Western blotting.

    Planar Chromatography:

    Article Title: A Novel Chemotherapy Combination to Enhance Proteotoxic Cell Death in Hepatocellular Carcinoma Experimental Models Without Killing Non-Cancer Cells
    Article Snippet: RCF was obtained from Hepion Pharmaceuticals (Edmonton, AB, Canada); Ixz (MLN2238 biologically active [#A4008], the MLN9708 prodrug [#A4007]), and QVD (#A1901) were obtained from APExBIO (Houston, TX, USA); cycloheximide (#239765), SAR405 (#533063), doxycycline (#D9891), polybrene (#TR-1003-G), 2-hydroxypropyl-b-cyclodextrin (#H5784) were obtained from Sigma-Aldrich (St. Louis, MO, USA); cyclosporin A (#BML-A195) was obtained from Enzo Life Sciences (Farmingdale, NY, USA); thapsigargin (#sc-24017) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); STF-083010 (#17370) and MKC-3946 (#19152) were obtained from Cayman Chemicals (Ann Arbor, MI, USA); and Coomassie blue (#20278), trypan blue (0.4%; #15250061), and puromycin (#J67236.8EQ) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. The human HCC cell lines Hep3B (#HB-8064), PLC/PRF/5 (#CRL-8024), and HepG2 (#HB-8065), and non-cancer cells EA.hy926 (EA, umbilical vein; #CRL-2922) and dermal fibroblasts (HDFs; #PCS-201-012) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and used within 6 months of resuscitation of the original cultures. .. HCC and EA cells were maintained in DMEM or EMEM (Thermo Fisher Scientific, ATCC) with 10% fetal bovine serum (R&D Systems, Minneapolis, MN, USA).

    Article Title: A Novel Chemotherapy Combination to Enhance Proteotoxic Cell Death in Hepatocellular Carcinoma Experimental Models Without Killing Non-Cancer Cells.
    Article Snippet: RCF was obtained from Hepion Pharmaceuticals (Edmonton, AB, Canada); Ixz (MLN2238 biologically active [#A4008], the MLN9708 prodrug [#A4007]), and QVD (#A1901) were obtained from APExBIO (Houston, TX, USA); cycloheximide (#239765), SAR405 (#533063), doxycycline (#D9891), polybrene (#TR-1003-G), 2-hydroxypropyl-bcyclodextrin (#H5784) were obtained from Sigma-Aldrich (St. Louis, MO, USA); cyclosporin A (#BML-A195) was obtained from Enzo Life Sciences (Farmingdale, NY, USA); thapsigargin (#sc-24017) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); STF-083010 (#17370) and MKC-3946 (#19152) were obtained from Cayman Chemicals (Ann Arbor, MI, USA); and Coomassie blue (#20278), trypan blue (0.4%; #15250061), and puromycin (#J67236.8EQ) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). .. The human HCC cell lines Hep3B (#HB-8064), PLC/PRF/5 (#CRL-8024), and HepG2 (#HB-8065), and non-cancer cells EA.hy926 (EA, umbilical vein; #CRL-2922) and dermal fibroblasts (HDFs; #PCS-201-012) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and used within 6 months of resuscitation of the original cultures. .. HCC and EA cells were maintained in DMEM or EMEM (Thermo Fisher Scientific, ATCC) with 10% fetal bovine serum (R&D Systems, Minneapolis, MN, USA).



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    SLC41A3 promotes HCC progression. (A) Western blot analysis confirming the knockdown efficiency of SLC41A3 in <t>Hep3B</t> and HuH7 cells transfected with shRNA targeting SLC41A3. (B) CCK-8 assay evaluating cell proliferation at specified time points after SLC41A3 silencing in HCC cells. (C) Colony formation ability of Hep3B and HuH7 cells was inhibited after SLC41A3 knockdown. (D) Transwell migration and invasion assays indicated impaired migration and invasion capabilities after SLC41A3 downregulation in both cell lines. (E) Wound healing assay showed reduced cell motility after SLC41A3 knockdown. Scale bar, 100 μm. Data are presented as the mean ± SD of three independent experiments. *** P < 0.001.
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    Image Search Results


    SLC41A3 promotes HCC progression. (A) Western blot analysis confirming the knockdown efficiency of SLC41A3 in Hep3B and HuH7 cells transfected with shRNA targeting SLC41A3. (B) CCK-8 assay evaluating cell proliferation at specified time points after SLC41A3 silencing in HCC cells. (C) Colony formation ability of Hep3B and HuH7 cells was inhibited after SLC41A3 knockdown. (D) Transwell migration and invasion assays indicated impaired migration and invasion capabilities after SLC41A3 downregulation in both cell lines. (E) Wound healing assay showed reduced cell motility after SLC41A3 knockdown. Scale bar, 100 μm. Data are presented as the mean ± SD of three independent experiments. *** P < 0.001.

    Journal: Frontiers in Immunology

    Article Title: Comprehensive characterization of SLC41A3 identifies it as an immune-related prognostic biomarker and therapeutic target in hepatocellular carcinoma

    doi: 10.3389/fimmu.2026.1861310

    Figure Lengend Snippet: SLC41A3 promotes HCC progression. (A) Western blot analysis confirming the knockdown efficiency of SLC41A3 in Hep3B and HuH7 cells transfected with shRNA targeting SLC41A3. (B) CCK-8 assay evaluating cell proliferation at specified time points after SLC41A3 silencing in HCC cells. (C) Colony formation ability of Hep3B and HuH7 cells was inhibited after SLC41A3 knockdown. (D) Transwell migration and invasion assays indicated impaired migration and invasion capabilities after SLC41A3 downregulation in both cell lines. (E) Wound healing assay showed reduced cell motility after SLC41A3 knockdown. Scale bar, 100 μm. Data are presented as the mean ± SD of three independent experiments. *** P < 0.001.

    Article Snippet: The human HCC cell lines Hep3B and Huh7 were purchased from Procell Biotechnology.

    Techniques: Western Blot, Knockdown, Transfection, shRNA, CCK-8 Assay, Migration, Wound Healing Assay

    Journal: bioRxiv

    Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

    doi: 10.1101/2025.11.27.691042

    Figure Lengend Snippet:

    Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

    Techniques: Concentration Assay

    Modulation of paraxanthine accumulation by known CYP1A2 effectors in HepG2 and Hep3B cells. Paraxanthine concentration was quantified by LC–MRM. Data are shown as mean ± SD from three independent experiments. A-B, Sulforaphane reduced paraxanthine accumulation in HepG2 and Hep3B cells. C-D , Galangin increased paraxanthine accumulation in both cell lines. E-F , 3-Methylcholanthrene (3-MC) also increased paraxanthine accumulation in both cell lines. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: bioRxiv

    Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

    doi: 10.1101/2025.11.27.691042

    Figure Lengend Snippet: Modulation of paraxanthine accumulation by known CYP1A2 effectors in HepG2 and Hep3B cells. Paraxanthine concentration was quantified by LC–MRM. Data are shown as mean ± SD from three independent experiments. A-B, Sulforaphane reduced paraxanthine accumulation in HepG2 and Hep3B cells. C-D , Galangin increased paraxanthine accumulation in both cell lines. E-F , 3-Methylcholanthrene (3-MC) also increased paraxanthine accumulation in both cell lines. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

    Techniques: Concentration Assay

    qPCR measurement of CYP1A2 mRNA levels in HepG2 (top panels) and Hep3B (bottom panels) cells after 24-hour treatments with A-B , caffeine and caffeine along with C-D, D,L-sulforaphane, E-F , 3-methylcholanthrene, or G-H , galangin at the indicated concentrations. Control cells received the vehicle only. Data are normalized to a housekeeping gene and expressed as the mean ± SD (n = 3) from three independent experiments. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: bioRxiv

    Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

    doi: 10.1101/2025.11.27.691042

    Figure Lengend Snippet: qPCR measurement of CYP1A2 mRNA levels in HepG2 (top panels) and Hep3B (bottom panels) cells after 24-hour treatments with A-B , caffeine and caffeine along with C-D, D,L-sulforaphane, E-F , 3-methylcholanthrene, or G-H , galangin at the indicated concentrations. Control cells received the vehicle only. Data are normalized to a housekeeping gene and expressed as the mean ± SD (n = 3) from three independent experiments. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

    Techniques: Control