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human hcc cell lines hep3b  (ATCC)


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    ATCC human hcc cell lines hep3b
    Human Hcc Cell Lines Hep3b, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human hcc cell lines hep3b/product/ATCC
    Average 98 stars, based on 1180 article reviews
    human hcc cell lines hep3b - by Bioz Stars, 2026-02
    98/100 stars

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    USP27X‐AS1 promoted hepatocellular carcinoma <t>(HCC)</t> proliferation, migration and invasion in vitro. (A) qRT‐PCR tested the efficacy of knockdown (left) or overexpression (right) of USP27X‐AS1 in HCC cell lines. (B) CCK‐8 assay of MHCC97H USP27X‐AS1 knockdown cell line <t>and</t> <t>HLF</t> USP27X‐AS1 overexpression cell line. (C and D) Representative images (C) and number (D) of colonies in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (E and F) Representative images (E) and positive cell number (F) of EdU assay in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (G and H) Representative images (G) and number (H) of migration or invasion cells in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (I and J) Representative images (I) and wound closure rate (J) of wound healing assay in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. Data and error bars are shown as mean ± SD of triplicate independent replicate experiments. * p < .05, ** p < .01, *** p < .001, ns: no significance. Data were analysed by paired Student's t ‐test (A, B, D, F, H and J).
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    Journal: bioRxiv

    Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

    doi: 10.1101/2025.11.27.691042

    Figure Lengend Snippet:

    Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

    Techniques: Concentration Assay

    Modulation of paraxanthine accumulation by known CYP1A2 effectors in HepG2 and Hep3B cells. Paraxanthine concentration was quantified by LC–MRM. Data are shown as mean ± SD from three independent experiments. A-B, Sulforaphane reduced paraxanthine accumulation in HepG2 and Hep3B cells. C-D , Galangin increased paraxanthine accumulation in both cell lines. E-F , 3-Methylcholanthrene (3-MC) also increased paraxanthine accumulation in both cell lines. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: bioRxiv

    Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

    doi: 10.1101/2025.11.27.691042

    Figure Lengend Snippet: Modulation of paraxanthine accumulation by known CYP1A2 effectors in HepG2 and Hep3B cells. Paraxanthine concentration was quantified by LC–MRM. Data are shown as mean ± SD from three independent experiments. A-B, Sulforaphane reduced paraxanthine accumulation in HepG2 and Hep3B cells. C-D , Galangin increased paraxanthine accumulation in both cell lines. E-F , 3-Methylcholanthrene (3-MC) also increased paraxanthine accumulation in both cell lines. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

    Techniques: Concentration Assay

    qPCR measurement of CYP1A2 mRNA levels in HepG2 (top panels) and Hep3B (bottom panels) cells after 24-hour treatments with A-B , caffeine and caffeine along with C-D, D,L-sulforaphane, E-F , 3-methylcholanthrene, or G-H , galangin at the indicated concentrations. Control cells received the vehicle only. Data are normalized to a housekeeping gene and expressed as the mean ± SD (n = 3) from three independent experiments. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: bioRxiv

    Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

    doi: 10.1101/2025.11.27.691042

    Figure Lengend Snippet: qPCR measurement of CYP1A2 mRNA levels in HepG2 (top panels) and Hep3B (bottom panels) cells after 24-hour treatments with A-B , caffeine and caffeine along with C-D, D,L-sulforaphane, E-F , 3-methylcholanthrene, or G-H , galangin at the indicated concentrations. Control cells received the vehicle only. Data are normalized to a housekeeping gene and expressed as the mean ± SD (n = 3) from three independent experiments. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

    Techniques: Control

    USP27X‐AS1 promoted hepatocellular carcinoma (HCC) proliferation, migration and invasion in vitro. (A) qRT‐PCR tested the efficacy of knockdown (left) or overexpression (right) of USP27X‐AS1 in HCC cell lines. (B) CCK‐8 assay of MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (C and D) Representative images (C) and number (D) of colonies in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (E and F) Representative images (E) and positive cell number (F) of EdU assay in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (G and H) Representative images (G) and number (H) of migration or invasion cells in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (I and J) Representative images (I) and wound closure rate (J) of wound healing assay in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. Data and error bars are shown as mean ± SD of triplicate independent replicate experiments. * p < .05, ** p < .01, *** p < .001, ns: no significance. Data were analysed by paired Student's t ‐test (A, B, D, F, H and J).

    Journal: Clinical and Translational Medicine

    Article Title: SP1‐activated USP27X‐AS1 promotes hepatocellular carcinoma progression via USP7‐mediated AKT stabilisation

    doi: 10.1002/ctm2.1563

    Figure Lengend Snippet: USP27X‐AS1 promoted hepatocellular carcinoma (HCC) proliferation, migration and invasion in vitro. (A) qRT‐PCR tested the efficacy of knockdown (left) or overexpression (right) of USP27X‐AS1 in HCC cell lines. (B) CCK‐8 assay of MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (C and D) Representative images (C) and number (D) of colonies in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (E and F) Representative images (E) and positive cell number (F) of EdU assay in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (G and H) Representative images (G) and number (H) of migration or invasion cells in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. (I and J) Representative images (I) and wound closure rate (J) of wound healing assay in MHCC97H USP27X‐AS1 knockdown cell line and HLF USP27X‐AS1 overexpression cell line. Data and error bars are shown as mean ± SD of triplicate independent replicate experiments. * p < .05, ** p < .01, *** p < .001, ns: no significance. Data were analysed by paired Student's t ‐test (A, B, D, F, H and J).

    Article Snippet: The China Center for Type Culture Collection (Wuhan, China) provided human HCC cell lines, including HLF, Hep3B, LM3, MHCC97H, HepG2 and PLC/PRF/5, along with the normal liver cell line THLE3.

    Techniques: Migration, In Vitro, Quantitative RT-PCR, Knockdown, Over Expression, CCK-8 Assay, EdU Assay, Wound Healing Assay

    USP27X‐AS1 promoted hepatocellular carcinoma (HCC) progression by AKT. (A–D) CCK‐8 detected the effect of USP27X‐AS1 knockdown (MHCC97H, LM3) or overexpression (HLF, Hep3B) on the proliferation of HCC cells. (E–H) The number of colonies of the above‐mentioned cell lines. (I–L) The number of EdU + cells of the above‐mentioned cell lines. (M–P) The number of migration and invasion cells of the above‐mentioned cell lines. (Q–T) The percentage of wound closure of the above‐mentioned cell lines. Data and error bars are shown as mean ± SD of triplicate independent replicate experiments. * p < .05, ** p < .01, *** p < .001, ns: no significance. Data were analysed by paired Student's t ‐test (A–T).

    Journal: Clinical and Translational Medicine

    Article Title: SP1‐activated USP27X‐AS1 promotes hepatocellular carcinoma progression via USP7‐mediated AKT stabilisation

    doi: 10.1002/ctm2.1563

    Figure Lengend Snippet: USP27X‐AS1 promoted hepatocellular carcinoma (HCC) progression by AKT. (A–D) CCK‐8 detected the effect of USP27X‐AS1 knockdown (MHCC97H, LM3) or overexpression (HLF, Hep3B) on the proliferation of HCC cells. (E–H) The number of colonies of the above‐mentioned cell lines. (I–L) The number of EdU + cells of the above‐mentioned cell lines. (M–P) The number of migration and invasion cells of the above‐mentioned cell lines. (Q–T) The percentage of wound closure of the above‐mentioned cell lines. Data and error bars are shown as mean ± SD of triplicate independent replicate experiments. * p < .05, ** p < .01, *** p < .001, ns: no significance. Data were analysed by paired Student's t ‐test (A–T).

    Article Snippet: The China Center for Type Culture Collection (Wuhan, China) provided human HCC cell lines, including HLF, Hep3B, LM3, MHCC97H, HepG2 and PLC/PRF/5, along with the normal liver cell line THLE3.

    Techniques: CCK-8 Assay, Knockdown, Over Expression, Migration