human hcc cell lines hep3b  (ATCC)

Journal: Frontiers in Immunology

Article Title: Comprehensive characterization of SLC41A3 identifies it as an immune-related prognostic biomarker and therapeutic target in hepatocellular carcinoma

doi: 10.3389/fimmu.2026.1861310

Figure Lengend Snippet: SLC41A3 promotes HCC progression. (A) Western blot analysis confirming the knockdown efficiency of SLC41A3 in Hep3B and HuH7 cells transfected with shRNA targeting SLC41A3. (B) CCK-8 assay evaluating cell proliferation at specified time points after SLC41A3 silencing in HCC cells. (C) Colony formation ability of Hep3B and HuH7 cells was inhibited after SLC41A3 knockdown. (D) Transwell migration and invasion assays indicated impaired migration and invasion capabilities after SLC41A3 downregulation in both cell lines. (E) Wound healing assay showed reduced cell motility after SLC41A3 knockdown. Scale bar, 100 μm. Data are presented as the mean ± SD of three independent experiments. *** P < 0.001.

Article Snippet: The human HCC cell lines Hep3B and Huh7 were purchased from Procell Biotechnology.

Techniques: Western Blot, Knockdown, Transfection, shRNA, CCK-8 Assay, Migration, Wound Healing Assay

Journal: bioRxiv

Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

doi: 10.1101/2025.11.27.691042

Figure Lengend Snippet:

Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

doi: 10.1101/2025.11.27.691042

Figure Lengend Snippet: Modulation of paraxanthine accumulation by known CYP1A2 effectors in HepG2 and Hep3B cells. Paraxanthine concentration was quantified by LC–MRM. Data are shown as mean ± SD from three independent experiments. A-B, Sulforaphane reduced paraxanthine accumulation in HepG2 and Hep3B cells. C-D , Galangin increased paraxanthine accumulation in both cell lines. E-F , 3-Methylcholanthrene (3-MC) also increased paraxanthine accumulation in both cell lines. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

doi: 10.1101/2025.11.27.691042

Figure Lengend Snippet: qPCR measurement of CYP1A2 mRNA levels in HepG2 (top panels) and Hep3B (bottom panels) cells after 24-hour treatments with A-B , caffeine and caffeine along with C-D, D,L-sulforaphane, E-F , 3-methylcholanthrene, or G-H , galangin at the indicated concentrations. Control cells received the vehicle only. Data are normalized to a housekeeping gene and expressed as the mean ± SD (n = 3) from three independent experiments. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

Techniques: Control